1.) Please quit out of both iTunes & Tuneup and go to the following site to download the appropriate version of TuneUp for your operating system: http://www.tuneupmedia.com/download.
2.) If you're on a Mac, double click the downloaded file and allow it to mount to your desktop. After that, a window should appear instructing you to drag the TuneUp application into your computer's Applications folder. If you're on a PC, simply double click the downloaded "exe" file to begin the installation process.
3.) Relaunch iTunes and TuneUp.
4.) If you have a paid license for TuneUp, this "trial" version will become the full "activated" version of TuneUp once you re-enter your Activation Code. After logging in, please locate the red "upgrade" button on the bottom of the TuneUp window, and enter your activation code in the space provided.